Anti-TOP1

Anti-TOP1
Item number Size Datasheet Manual SDS Delivery time Quantity Price
A302-589A-T 20 µl (2 blots) -

3 - 9 business days*

131.00€
A302-589A-M 100 µl (10 blots) -

3 - 9 business days*

313.00€
A302-589A 100 µl (20 µg) -

3 - 9 business days*

465.00€
 
Protein function: Releases the supercoiling and torsional tension of DNA introduced during the... more
Product information "Anti-TOP1"
Protein function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. [The UniProt Consortium]
Keywords: Anti-TOP1, EC=5.99.1.2, Anti-DNA topoisomerase I, Anti-DNA topoisomerase 1
Supplier: Bethyl Laboratories
Supplier-Nr: A302-589A

Properties

Application: WB, IP
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human (Expected: Rat)
Immunogen: synthetic peptide. The epitope recognized by A302-589A-T maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
Format: Antigen Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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