Anti-TIM-1 (T Cell Immunoglobin Domain and Mucin Domain Protein 1, TIM 1, TIM1, TIMD 1, TIMD1, TIMD-

TIM-1, also known as HAVcr1 (C Hepatitis A virus cellular receptor 1) and KIM-1 (Kidney Injury Molecule 1), is a 60-100kD member of the TIM family of immune regulating adhesion proteins. The mature molecule is a type I transmembrane glycoprotein that contains a 214aa extracellular domain (ECD) and a 51aa cytoplasmic tail. The ECD contains one Ig-like V-type domain, a Ser/Thr-rich mucin region, and multiple glycosylation sites. TIM-1 is involved in the development of Th2-biased immune responses, and the presence of particular TIM-1 mutations correlates with the susceptibility to allergic disease. TIM-1 binds TIM-4 and serves as the receptor for the hepatitis A virus. It is also upregulated in renal epithelial cells following tissue injury. The extracellular domain of TIM-1 can be shed and serves as an indicator of kidney tubule damage. Within the ECD, rat TIM-1 shares 81% and 51% aa sequence identity with mouse and human TIM-1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human or mouse brain tissue lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.