Anti-Teneurin-1 (Ten-1, Tenascin-M1, Ten-m1, Protein Odd Oz/ten-m homolog 1, ODZ1, TNM1)

Teneurin-1(also Ten-m1, tenascin-M1, and Ten-m/Odz1) is a 250-300kD member of the tenascin family, teneurin subfamily of transmembrane (TM) molecules. It is a covalently-linked homodimer that is expressed in both embryonic and adult neurons, among which are cerebellar granule cells and CA2 pyramidal hippocampal neurons. Teneurin-1 appears to promote neurite outgrowth and mediate cell-to-cell adhesion via homophilic interactions. Human teneurin-1 is a 2725aa type II TM glycoprotein. It contains a 324aa cytoplasmic region (aa1-324) that contains an NLS (aa62-65), plus a 2380aa extracellular domain (ECD). The ECD possesses eight sequential EGF-like domains (aa528-796), five NHL repeats, each of which form a b-propeller (aa1194-1524), and 23 YD/TyrAsp-containing repeats that bind carbohydrates. Cleavage at the N-terminus generates an initial 65kD membrane-spanning fragment, followed by TM cleavage that generates a 45kD cytosolic fragment. C-terminal cleavage generates a short 5kD 41aa peptide (aa2682-2722) termed TCAP-1 that shows bioactivity. One splice variant shows a deletion of (aa1232-1239). Over (aa1-317, human Teneurin-1 shares 96% aa identity with mouse Teneurin-1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.