Anti-Syntaxin (SP-6, HPC1, P35 1, STX1, STX1A, STX1B, STX2, STX3)

Syntaxins are a family of membrane integrated Q-SNARE proteins participating in exocytosis (1). They possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. This SNARE domain binds to synaptobrevin and SNAP-25 to form the SNARE complex (2, 3). Support of syntaxin's involvement in vesicular transport and neurotransmission includes the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (4). Syntaxin also provides the target pf clostridial neurotoxin C1, which blocks neurotransmitter release (5). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, a-SNAP and NSF (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.