Anti-SMN Monoclonal Antibody (Clone 4B7)

The survival motor neuron (SMN) protein is a ubiquitously expressed, multifunctional protein with roles in a variety of cellular processes, including small nuclear ribonucleoprotein (snRNP) and spliceosome assembly, translation, cytoskeletal dynamics, and stress granule formation. It is a 294-amino acid protein encoded by SMN1 in humans and is comprised of an N-terminal basic/lysine-rich domain that interacts with RNA and Gemin-2, a central Tudor domain (Cay-14136) that functions in a variety of protein-protein interactions, a proline-rich region, and a C-terminal YG box that facilitates SMN oligomerization. Humans also have a second centromeric copy of the gene, called SMN2, that is the result of duplication and inversion and primarily produces a truncated isoform, SMNDelta7, which is less stable, but can also produce small amounts of SMN. Knockout of Smn is embryonic lethal in mice. In humans, mutations in SMN1 are associated with spinal muscular atrophy (SMA), a neuromuscular disorder characterized by degeneration of alpha motor neurons in the spinal cord and muscle weakness. Cayman's SMN Monoclonal Antibody can be used for immunocytochemistry (ICC) and Western blot (WB) applications. The antibody recognizes SMN protein at approximately 35 kDa from human, mouse, and rat samples.Synonyms: Gemin-1, Survival Motor Neuron Protein. Immunogen: Recombinant human His-tagged SMN protein expressed in E. coli. The epitope recognized by the 4B7 clone binds to the region encoded by exon 1.. Clone Designation: 4B7. MW: ~35 kDa. Formulation: (Request formulation change), 100 µl of protein G-purified monoclonal antibody. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Mouse. Isotype: IgG1. Applications: ICC, WB.