Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
S7982-03F.100 | 100 µl | - | - |
3 - 19 business days* |
682.00€
|
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by... more
Product information "Anti-SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-"
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms, however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200, Western Blot: 1:2000, Immunoprecipitation: 1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-SMC protein 3, Anti-Chromosome-associated polypeptide, Anti-Chondroitin sulfate proteoglycan 6, Anti-Structural maintenance of chromosomes protein 3, Anti-Basement membrane-associated chondroitin proteoglycan |
Supplier: | United States Biological |
Supplier-Nr: | S7982-03F |
Properties
Application: | IF, IP, WB |
Antibody Type: | Monoclonal |
Clone: | D47B5 |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey, mouse, rat |
Immunogen: | Synthetic peptide corresponding to residues surrounding Arg132 of human SMC3. |
Format: | Supernatant |
Database Information
KEGG ID : | K06669 | Matching products |
UniProt ID : | Q9UQE7 | Matching products |
Gene ID | GeneID 9126 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed