Anti-SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-

Anti-SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-
Item number Size Datasheet Manual SDS Delivery time Quantity Price
S7982-03F.100 100 µl - -

3 - 19 business days*

682.00€
 
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by... more
Product information "Anti-SMC3 (Structural Maintenance of Chromosomes Protein 3, SMC Protein 3, SMC-3, Basement Membrane-"
The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms, however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200, Western Blot: 1:2000, Immunoprecipitation: 1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-SMC protein 3, Anti-Chromosome-associated polypeptide, Anti-Chondroitin sulfate proteoglycan 6, Anti-Structural maintenance of chromosomes protein 3, Anti-Basement membrane-associated chondroitin proteoglycan
Supplier: United States Biological
Supplier-Nr: S7982-03F

Properties

Application: IF, IP, WB
Antibody Type: Monoclonal
Clone: D47B5
Conjugate: No
Host: Rabbit
Species reactivity: human, monkey, mouse, rat
Immunogen: Synthetic peptide corresponding to residues surrounding Arg132 of human SMC3.
Format: Supernatant

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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