Anti-SIK2 / Salt-inducible kinase 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SIK2 antibody detects Salt-inducible kinase 2, a serine/threonine kinase that regulates metabolism, stress response, and cell cycle progression. The UniProt recommended name is Salt-inducible kinase 2 (SIK2). This enzyme is part of the AMP-activated protein kinase (AMPK) family and functions as a signaling integrator controlling glucose metabolism, lipolysis, and mitotic regulation in both normal and tumor cells.Functionally, SIK2 antibody identifies a 926-amino-acid cytoplasmic protein containing an N-terminal kinase domain, an autoinhibitory region, and regulatory phosphorylation sites. SIK2 phosphorylates transcriptional coactivators such as CRTC2 and HDAC4, repressing cAMP-response element-binding protein (CREB)-mediated transcription. Through this action, SIK2 coordinates the response to metabolic hormones and nutrient availability, ensuring energy balance under varying physiological conditions.The SIK2 gene is located on chromosome 11q23.3 and is expressed in adipose tissue, skeletal muscle, and brain. It plays a critical role in insulin signaling by modulating the expression of gluconeogenic and lipogenic enzymes. In adipocytes, SIK2 regulates hormone-sensitive lipase activity and lipid mobilization. It also influences mitochondrial biogenesis and oxidative metabolism, linking it to overall energy homeostasis. In the nervous system, SIK2 participates in neuronal differentiation and synaptic plasticity through phosphorylation of CREB cofactors.In the cell cycle, SIK2 acts as a mitotic regulator. It localizes to centrosomes and controls spindle orientation and cytokinesis. Overexpression of SIK2 promotes centrosome separation and mitotic progression, while its inhibition disrupts mitosis and sensitizes cancer cells to chemotherapeutic agents. Elevated SIK2 expression has been reported in ovarian and breast cancers, where it supports cell proliferation and metabolic adaptation.SIK2 antibody is widely used in studies of metabolism, kinase signaling, and cancer biology. It is suitable for western blotting, immunofluorescence, and kinase assay applications to detect endogenous SIK2 and phosphorylated substrates. In metabolic research, this antibody aids in evaluating AMPK-related signaling, energy regulation, and hormonal control of gene expression. In oncology, SIK2 serves as a potential therapeutic target due to its dual role in metabolism and mitotic control.Structurally, SIK2 contains a conserved activation loop that undergoes phosphorylation by upstream kinases such as LKB1, essential for catalytic activation. Its activity is negatively regulated by cAMP-dependent protein kinase (PKA)-mediated phosphorylation, which induces 14-3-3 protein binding and cytoplasmic sequestration. NSJ Bioreagents provides SIK2 antibody reagents validated for use in metabolic, signal transduction, and mitotic regulation research.