Anti-SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobuli

SIGIRR is a type I transmembrane protein belonging to the IL-1 receptor superfamily. It has a single extracellular Ig-like domain and conserved motifs in its cytoplasmic domain that are characteristic of the IL-1 receptor and Toll-like receptor families. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using human PBMCs., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. After blocking, add 10ul of S1013-68G to 1-2.5 x 10e5 cells and incubate for 30 minutes at RT., 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (Catalog# F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer., 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.