Anti-RUNX3 (Runt-related Transcription Factor 3, Acute Myeloid Leukemia 2 Protein, AML2, CBFA3, CBF-

RUNX3, also called CBFA3, AML-2 or PEBP2-aC, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-b. The runt domain (aa54-186) is required for DNA binding, while a Pro/Ser/Thr-rich region (aa191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19aa sequence in place of the N-terminal 5aa of isoform 1. The 415aa human RUNX3 shows 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.