Anti-Renin, Pro/Mature

Renin is secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin. The 406aa precursor includes a signal peptide (aa 1-23), a propeptide (aa 24-66), and a mature chain (aa 67-406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat. Applications: Suitable for use in Immunoprecipitation, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml, Western Blot: 1ug/ml under non-reducing conditions only, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.