Anti-PLXNB1 (Plexin B1, KIAA0407, MGC149167, PLXN5, Semaphorin Receptor SEP, SEP) (PE)

PLXNB1 is a 300kD member of the B subfamily, plexin family of semaphorin receptors. Mature human PLXNB1 is a 2116aa type I transmembrane (TM) glycoprotein that contains a 1471aa extracellular domain (ECD) and a 612aa cytoplasmic region. The ECD contains one semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200kD a-chain (aa20-1305) and a 100kD TM b-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. PLXNB1 is a receptor for CD100/Sema4D. It forms a receptor complex with neuropilins, MET, and EGFR2. Multiple splice variants are known. One shows a deletion of aa689-871. A second shows a 53aa substitution for aa677-2135. And a third shows a 53aa substitution for the 53aa between aa677-729. Over aa35-150, human PLXNB1 is 90% and 97% aa identical to dog and mouse PLXNB1, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells. Validated for use with Jurkat human acute T cell leukemia cell line, Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with NS0 cells transfected with human PLXNB1. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.