Anti-Plexin B1 (PLXNB1, KIAA0407, MGC149167, Plexin-B1, PLEXIN-B1, PLXN5, Semaphorin Receptor SEP, S

Plexin B1 is a 300kD member of the B subfamily, plexin family of semaphorin receptors. Mature human Plexin B1 is a 2116aa type I transmembrane (TM) glycoprotein that contains a 1471aa extracellular domain (ECD) and a 612aa cytoplasmic region. The ECD contains one semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200kD alpha-chain (aa20-1305) and a 100kD TM beta-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. PlexinB1 is a receptor for CD100/Sema4D. It forms a receptor complex with neuropilins, MET, and EGFR2. Multiple splice variants are known. One shows a deletion of aa689-871. A second shows a 53aa substitution for aa677-2135. And a third shows a 53aa substitution for the 53aa between aa677-729. over aa35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in 100-200ul, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human Plexin B1 (aa35-150). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.