Anti-PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev 107 Protei

PLA2G16 (Group 16 phospholipase A2, also HREV107 and AdPLA) is a Ca++-dependent 17-18kD member of the H-rev107 family of molecules. It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids. The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels. This results in decreased hormone-sensitive lipase activity and increased adiposity. Human PLA216 is 16211aa in length. It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa134-154. The enzymatic activity resides in His23 and Cys113. There is one potential isoform that shows a 20aa substitution for the first four amino acids. Over aa12-132, human PLA2G16 shares 88% aa identity with mouse PLA2G16. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA:0.5-1ug/ml, Western Blot:1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.