Anti-PKC delta/theta / PRKCD/PRKCQ, clone 31P77

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. PKC delta/theta antibody detects protein kinase C delta and protein kinase C theta, encoded by the PRKCD and PRKCQ genes. PKC delta is also called nPKC delta, while PKC theta is known as nPKC theta. Both belong to the novel PKC subfamily, which are activated by diacylglycerol but not calcium. PKC delta is broadly expressed and regulates apoptosis, proliferation, and differentiation, while PKC theta is highly enriched in T cells and is critical for T cell receptor signaling and immune synapse formation. Detecting both isoforms with a single antibody provides a versatile approach for studying PKC signaling across systems.PKC delta/theta antibody is widely applied in immunology, cancer biology, and signal transduction studies. PKC delta acts as a tumor suppressor or promoter depending on context, influencing apoptosis, DNA damage responses, and cell cycle regulation. PKC theta is essential for T cell activation, NF kappa B signaling, and cytokine production. By detecting both isoforms, researchers can evaluate overlapping and distinct functions in immunity, cancer, and tissue homeostasis.Applications for PKC delta/theta antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect PKC isoforms in immune and cancer cell lysates, immunohistochemistry maps expression in tissues, and immunofluorescence highlights subcellular localization at membranes and cytoskeleton. These methods support detailed characterization of PKC signaling dynamics.PKC delta contributes to apoptosis in response to DNA damage and oxidative stress, while PKC theta mediates survival signals in T cells. Dysregulated activity of either isoform contributes to autoimmunity, cancer progression, and cardiovascular disease. By applying PKC delta/theta antibody, scientists can examine how combined PKC signaling influences disease outcomes and therapeutic responses.PKC isoforms are therapeutic targets, with inhibitors and modulators under development for cancer and immune disorders. Monitoring PKC delta and PKC theta expression provides biomarkers for pathway activity. PKC delta/theta antibody from NSJ Bioreagents provides strong specificity for these isoforms, supporting both mechanistic and translational research.