Anti-PIK3C2A / Phosphoinositide 3-kinase-C2-alpha

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PIK3C2A antibody detects Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing alpha polypeptide, a lipid kinase that regulates membrane trafficking, signal transduction, and cytoskeletal organization. Encoded by the PIK3C2A gene on chromosome 11p15.5, this enzyme belongs to the class II phosphoinositide 3-kinase (PI3K) family and phosphorylates phosphatidylinositol and phosphatidylinositol 4-phosphate to generate phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2). Through these lipid intermediates, PIK3C2A coordinates endocytosis, vesicle trafficking, and cell polarity regulation.PIK3C2A contains a catalytic kinase domain, a C2 lipid-binding domain, and clathrin-binding regions that localize it to the plasma membrane and endosomes. Its enzymatic activity is essential for clathrin-mediated endocytosis and autophagosome formation. PIK3C2A also modulates signaling pathways downstream of receptor tyrosine kinases and integrins by influencing AKT activation and cytoskeletal reorganization.The PIK3C2A antibody is widely used in cell signaling, membrane biology, and cancer research to study phosphoinositide metabolism, vesicle formation, and kinase regulation. Western blot analysis identifies a 180-190 kilodalton band corresponding to PIK3C2A, while immunofluorescence reveals punctate staining in endosomal and plasma membrane compartments. This antibody enables the analysis of lipid signaling networks that control intracellular transport and cellular morphology.Loss or mutation of PIK3C2A has been linked to congenital cataracts, vascular defects, and impaired cilia formation due to disrupted membrane trafficking. Conversely, overexpression contributes to tumorigenesis through altered PI3K signaling and enhanced migration. The PIK3C2A antibody provides a robust tool for investigating lipid kinase function in physiology and disease. NSJ Bioreagents validates this antibody for western blotting and immunofluorescence, ensuring accuracy for research into phosphoinositide and trafficking pathways.