Anti-PI3 Kinase Class II Alpha (Class II Phosphatidylinositol 3-kinases, PI3KCII-alpha)

Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II a generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II a is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II a regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II a also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II a has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II a is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.