Anti-PDE8A / Phosphodiesterase 8A

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. PDE8A antibody detects Phosphodiesterase 8A, an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) to AMP, thereby regulating intracellular signaling pathways mediated by this second messenger. PDE8A belongs to the type 8 family of cyclic nucleotide phosphodiesterases, which are distinct in their insensitivity to common PDE inhibitors such as IBMX. The PDE8A antibody is widely used in studies of hormonal regulation, metabolism, and immune signaling, where precise modulation of cAMP levels is critical.PDE8A is encoded by the PDE8A gene located on human chromosome 15q25.3. The enzyme is approximately 93 kilodaltons and contains a conserved catalytic domain flanked by unique N-terminal and C-terminal regulatory regions. PDE8A is expressed in various tissues, including adrenal gland, heart, testis, and immune cells, where it contributes to compartmentalized cAMP degradation and pathway specificity. Its high substrate affinity and resistance to standard inhibitors make it a key regulator of localized signaling domains.The PDE8A antibody detects multiple isoforms ranging from 85-100 kilodaltons on western blot and reveals cytoplasmic and perinuclear staining in immunofluorescence assays. PDE8A associates with A-kinase anchoring proteins (AKAPs) and participates in cAMP microdomain organization, influencing downstream activation of protein kinase A (PKA) and exchange proteins activated by cAMP (EPACs). In immune cells, PDE8A modulates T-cell receptor signaling and cytokine production, contributing to immune tolerance and inflammatory regulation.Functional studies indicate that PDE8A influences steroidogenesis, insulin secretion, and energy balance by controlling cAMP-mediated transcriptional events. Knockdown or pharmacological inhibition enhances cAMP signaling and affects processes such as sperm motility, cortisol synthesis, and cardiac contractility. Altered PDE8A expression has been linked to autoimmune diseases, infertility, and metabolic disorders.PDE8A also plays roles in cancer biology, where its dysregulation affects cell proliferation and migration through altered PKA activity and transcriptional responses. NSJ Bioreagents provides a validated PDE8A antibody optimized for western blot, flow cytometry, and enzyme localization assays, enabling precise exploration of cyclic nucleotide signaling, endocrine regulation, and immune modulation.