Anti-Parvin, alpha

Alpha-Parvin (parvus [Latin for "small"]-A/ alpha, also actopaxin and PARV-A) is a 53kD member of the parvin family, alpha-actinin superfamily of proteins. It is widely expressed, and participates in actin filament assembly that precedes cell migration. Human PARVA is 372aa in length. It contains two CH/calponin homology domains (aa99-202 and 265-369), two NLSs , (aa19-26 and 38-40), and a recently identified paxillin binding sequence (aa248-256). Multiple phosphorylation sites occur between Ser4 and Thr16 that are apparently utilized. PARVA is likely to exist as a dimer. There are three isoform variants of PARVA. All three contain an alternate start site at Met41, with one also showing a 21aa substitution for aa239-372, and a second also showing a 46aa substitution for aa 134-372, respectively. Over aa46-133, human and mouse PARVA share 95% aa identity. , , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.