Anti-p70 S6K / RPS6KB1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. p70 S6K antibody detects Ribosomal protein S6 kinase beta-1, encoded by the RPS6KB1 gene on chromosome 17q23.1. p70 S6K antibody is widely applied in studies of cell growth, protein synthesis, and cancer biology. p70 S6K is a serine/threonine kinase activated by mTORC1 that phosphorylates the ribosomal protein S6, promoting translation of mRNAs encoding ribosomal and elongation factors. This kinase is a central effector of the PI3K-Akt-mTOR signaling pathway.Structurally, p70 S6K is a ~70 kDa protein with a conserved kinase catalytic domain, autoinhibitory domain, and multiple phosphorylation sites. Activation requires phosphorylation at Thr389 by mTORC1, as well as additional phosphorylation by PDK1 and MAPK pathways. Alternative splicing yields isoforms p70 and p85, which differ in N-terminal extensions and subcellular localization.Functionally, p70 S6K stimulates protein synthesis, cell cycle progression, and growth by enhancing translation efficiency. It regulates feedback inhibition of insulin receptor signaling and coordinates energy availability with biosynthetic demands. Researchers employ p70 S6K antibody to investigate translational control, mTOR signaling, and growth regulation.Clinically, p70 S6K is implicated in metabolic disorders and cancer. Hyperactivation of mTOR-S6K signaling contributes to obesity, insulin resistance, and type 2 diabetes. In oncology, RPS6KB1 amplification and overactivation drive tumorigenesis, supporting proliferation and survival. Pharmacological inhibitors of S6K and mTOR are under development as anti-cancer and anti-metabolic disease therapies. NSJ Bioreagents supplies p70 S6K antibody for signaling and cancer research.Experimentally, p70 S6K antibody is used in western blotting to detect the ~70 kDa protein, in immunohistochemistry to analyze tumor expression, and in immunofluorescence to examine subcellular localization. Phospho-specific antibodies complement p70 S6K antibody to study activation status.