Anti-p53 / TP53, clone rIMX25

0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide. Tumor Suppressor p53 antibody recognizes Tumor protein 53, a nuclear transcription factor encoded by the TP53 gene and widely known as p53, one of the most critical tumor suppressors in human cancer biology. Tumor protein 53 functions as a central regulator of genomic stability by controlling transcriptional programs involved in cell cycle arrest, DNA repair, apoptosis, and senescence. Under normal physiological conditions, p53 protein levels are maintained at low abundance through MDM2 mediated ubiquitination and proteasomal degradation, allowing regulated cell proliferation and tissue homeostasis.In response to cellular stress such as DNA damage, oncogene activation, hypoxia, or oxidative injury, p53 becomes stabilized through post translational modifications including phosphorylation and acetylation. Stabilized p53 accumulates in the nucleus, where it binds specific DNA response elements and activates transcription of downstream target genes such as CDKN1A, BAX, and PUMA. Through these pathways, Tumor protein 53 halts cell cycle progression to permit DNA repair or initiates apoptosis when genomic damage is beyond repair. This stress responsive checkpoint mechanism is essential for preventing propagation of genetically unstable cells and suppressing malignant transformation.Mutations in TP53 are among the most common genetic alterations observed in human cancers. Many tumor associated mutations produce a stable but functionally impaired p53 protein that accumulates within the nucleus, often resulting in strong nuclear immunoreactivity in tumor tissues. In some contexts, mutant p53 may acquire gain of function properties that contribute to tumor progression, invasion, and resistance to therapy. Because of its pivotal role in oncogenesis, p53 antibody is widely used in research and diagnostic studies focused on tumor classification and molecular pathology.Tumor protein 53 is predominantly localized to the nucleus, consistent with its role as a transcription factor, although cytoplasmic staining may be observed depending on mutation status and cellular context. The recombinant mouse monoclonal antibody clone rIMX25 is designed to detect p53 protein expression in research applications evaluating tumor suppressor pathways, DNA damage responses, and cell cycle regulation. As a recombinant monoclonal antibody, clone rIMX25 supports consistent detection of TP53 in normal and neoplastic tissues.