Anti-Nuclear Receptor LXR alpha, Ligand Binding Domain (NR1H3, RLD-1), clone 5L2

Human Liver X Receptor alpha (LXRa, RLD-1, NR1H3) is a 50kD member of the NR1/LXR subfamily of the nuclear hormone receptor family. It is tissue-specific (liver and macrophages) and forms a heterodimer with RXR. LXR is regulated by oxysterols such as 24,25 epoxycholesterol and 24-hydroxycholesterol. LXR activation reduces free cholesterol in macrophages and protects them from its cytotoxic effects. Human LXR alpha shares 62% amino acid identity to LXR beta, an analagous molecule that is the product of a separate gene. Human LXR alpha has at least two isoforms. One isoform is full-length at 447 amino acids while the other shows a 60 amino acid deletion between aa237-296. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA (Direct): 0.2ug/ml, Western Blot: 1ug/ml under reducing conditions, Immunohistochemistry: 20-40ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Produced in BALB/c mouse ascites inoculated with a hybridoma of spleen cells of a BALB/c mouse immunized with recombinant human LXR alpha (aa164-447) and mouse myeloma cells (NS-1). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.