Anti-Notch1, intracellular domain (Notch Homolog Protein 1, hN1, Translocation-associated Notch Prot

Anti-Notch1, intracellular domain (Notch Homolog Protein 1, hN1, Translocation-associated Notch Prot
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N5375-01D.200 200 µl - -

3 - 19 business days*

863.00€
 
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the... more
Product information "Anti-Notch1, intracellular domain (Notch Homolog Protein 1, hN1, Translocation-associated Notch Prot"
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20000-1:100000, Western Blot: 1:500-1:1100, Immunohistochemistry (paraffin): 1:100, Optimal dilutions to be determined by the researcher. Positive Control: HeLa Cell Lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-NICD, Anti-TAN1, Anti-Notch 1, Anti-Notch 1 intracellular domain, Anti-Notch 1 extracellular truncation, Anti-Neurogenic locus notch homolog protein 1, Anti-Translocation-associated notch protein TAN-1
Supplier: United States Biological
Supplier-Nr: N5375-01D

Properties

Application: ELISA, IHC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human
Immunogen: Synthetic peptide from human Notch 1. Epitope: Intracellular. Cellular Localization: Cell membrane, Single-pass type I membrane protein.
Format: Serum

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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