Anti-NOTCH1, Cleaved (Neurogenic Locus Notch Homolog Protein 1, Notch 1, hN1, Translocation-associat

The Notch protein family contains a group of highly conserved single-pass, ligand-activated transmembrane receptors involved in cell fate selection and maintenance of progenitors during development. These proteins also function as transcription factors. Notch proteins mainly consist of an extra cellular domain containing tandem EGF-repeats and three lin-12/Notch repeats, a transmembrane domain and an intracellular domain containing six cdc1/SW16/ankyrin repeats, RAM motif, nuclear localization signals and a C-terminal OPA/PEST region. NOTCH1, a mammalian homolog of Drosophila Notch, is an important member of this family. Physiological functions influenced by NOTCH proteins include maintenance of hematopoietic stem cells, T-cell development, neuronal function and development, angiogenesis, arterial and ventricular development and differentiation, cardiac valve homeostasis, cell lineage specification of endocrine and exocrine pancreas, development of actin cytoskeleton and bone regeneration. Abnormal Notch signaling has been associated with several diseases including cancer, leukemia, melanoma, T-ALL, CADASIL, Multiple Sclerosis, Tetralogy of Fallot and Alagille Syndrome. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 3-5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.