Anti-Neurocan (Neurocan Core Protein, Chondroitin Sulfate Proteoglycan 3, Ncan, Cspg3) (Biotin)

Neurocan (Neural proteoglycan, also CSPG3) is a secreted, 300-350kD proteoglycan member of the lectican family of molecules. It is expressed by neurons and astrocytes, and has multiple binding partners, including heparin, FGF2, HB-CAM, glypican-1, syndecan-3, and tenascin-C plus -R. Its expression is highest during development where it regulates neuronal migration and neurite outgrowth. Mature mouse Neurocan is 1246aa in length. It is highly modular, containing a V-type Ig-like domain (aa43-157), two link modules (aa159-356), two EGF-like domains (aa960-1034), a C-type lectin region (aa1036-1165), and a Sushi domain (aa1165-1225). Cleavage occurs between Met643 and Leu644, generating a 621aa, 170-190kD N-terminal fragment, and a 625aa, 180kD C-terminal fragment. Without proteoglycan adducts, SDS-page shows full-length Neurocan to be 245-260kD in size, with the N-terminal fragment 130kD, and the C-terminal fragment 150kD in size. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.