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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
N0577-15A.100 | 100 µg | - | - |
3 - 19 business days* |
916.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1, also RPF1) is a... more
Product information "Anti-NEDD4 (E3 Ubiquitin-protein Ligase NEDD4, Cell Proliferation-inducing Gene 53 Protein, Neural P"
NEDD4 (Neural precursor cell-expressed developmentally down-regulated gene 4-1, also RPF1) is a 115-120kD member of the HECT E3 ubiquitin ligase family of proteins. The NEDD4 gene is widely expressed, and serves as a complement to a related gene product termed NEDD4-2/NEDD4L. Both molecules ubiquitinate multiple proteins. NEDD4-1 acts preferentially on PTEN, tumor suppressors and endocytic proteins, while NEDD4-2 acts preferentially on membrane transporters. Human NEDD4-1 is 900aa in length and contains one PKC region (aa20-124), four WW domains (aa196-505) and one E3 HECT domain (aa543-897). Caspase cleavage after Asp197 generates a 95kD C-terminal, and smaller but variable sized N-terminal fragment. There are multiple splice forms. Two show a 516aa substitution for aa1-169 and 1-97, respectively, while a third shows the latter substitution coupled to a deletion of aa170-185. Over aa211-349, human NEDD4-1 shares 83% and 72aa identity to mouse and rat NEDD4-1 and less than 25aa identity with human NEDD4-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 25ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | EC=6.3.2.-, Anti-E3 ubiquitin-protein ligase NEDD4, Anti-Cell proliferation-inducing gene 53 protein, Anti-Neural precursor cell expressed developmentally down-regulated protein 4 |
Supplier: | United States Biological |
Supplier-Nr: | N0577-15A |
Properties
Application: | WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Sheep |
Species reactivity: | human, rat |
Immunogen: | E. coli-derived, recombinant human NEDD4 (aa211-349, Accession # P46934). |
Format: | Affinity Purified |
Database Information
KEGG ID : | K10591 | Matching products |
UniProt ID : | P46934 | Matching products |
Gene ID | GeneID 4734 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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