Anti-MYL2 / Myosin regulatory light chain 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. MYL2 antibody detects myosin regulatory light chain 2, a contractile protein critical for cardiac and skeletal muscle contraction. The UniProt recommended name is Myosin regulatory light chain 2 (MYL2). This 166-amino-acid protein binds to the neck region of myosin heavy chain, stabilizing the lever-arm structure and modulating actin-activated ATPase activity during force generation.Functionally, MYL2 antibody identifies a calcium-binding protein that undergoes phosphorylation by myosin light chain kinase (MLCK) at serine 15. Phosphorylation increases calcium sensitivity and enhances contractile force, playing a vital role in excitation-contraction coupling. MYL2 expression is strongest in cardiac ventricles, distinguishing it from atrial isoforms such as MYL7. Its presence marks ventricular myocytes in both mature and differentiating cardiac tissue.The MYL2 gene resides on chromosome 12q24.11 and encodes a regulatory component of the myosin complex. Mutations in MYL2 have been associated with familial hypertrophic cardiomyopathy, left ventricular dysfunction, and sudden cardiac death. These variants alter protein folding or phosphorylation sites, impairing actomyosin cross-bridge cycling and contractile mechanics.In muscle physiology, MYL2 acts as a fine-tuner of myosin motor function, adjusting contraction kinetics through dynamic phosphorylation. In developmental studies, MYL2 serves as a ventricular differentiation marker, while in pathology it reflects remodeling in cardiac hypertrophy and failure. Experimental models using MYL2 antibody provide insights into sarcomere assembly, cardiomyocyte maturation, and myofibrillar alignment.MYL2 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect cardiac myosin light chain expression. It is extensively used in cardiovascular research, muscle biology, and regenerative medicine to monitor contractile protein regulation. NSJ Bioreagents offers validated MYL2 antibody reagents optimized for studies of cardiac physiology and disease.Structurally, MYL2 belongs to the EF-hand calcium-binding family and interacts with the IQ motif of myosin heavy chains. Its N-terminal domain modulates ATP hydrolysis and contributes to sarcomeric order. This antibody enables precise detection of MYL2 to study contractile regulation, disease mechanisms, and cardiomyocyte differentiation.