Anti-Macrophage Colony Stimulating Factor Receptor (M-CSFR, CSF-1) (APC)

M-CSF R (macrophage colony stimulating factor receptor, c-fms) is a 165kD, 977aa type I membrane glycoprotein. It is a member of the type III subfamily of receptor tyrosine kinases that contain five extracellular immunoglobulin-like domains and a cytosolic split kinase domain. M-CSF receptor is expressed primarily on monocytes, macrophages, dendritic cells, and stem cells, and regulates lineage decisions and maturation of these cells. The mouse M-CSF R extracellular domain shares >99% aa identity with rat and 60-63% aa identity with human, dog, cat and cow M-CSF R. It can be released by TACE/ADAM17 cleavage. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 cells using muse reticulum cell sarcoma macrophage cell line, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from a rat immunized with purified NS0-derived recombinant mouse M-CSF R (aa20-511). Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.