Anti-LPP (Lipoma-preferred Partner, LIM Domain-containing Preferred Translocation Partner in Lipoma)

Human LIM domain-containing preferred translocation partner in lipoma (LPP) is an 80kD intracellular protein that contains an N-terminal Pro-rich region aa41-370 followed by three tandem LIM domains aa414-603. It localizes to focal adhesion plaques where it regulates junction assembly and cytoskeleton remodeling through interactions with a-Actinin, VASP, SCRIB, Supervillin, and Palladin. In the nucleus, LPP coactivates the transcription factor PEA3 and protects telomeres from DNA damage. LPP is a frequent target of chromosomal translocations with HMG-A2 in lipoma. Within aa138-261, human LPP shares 90% and 77% aa sequence identity with mouse and rat LPP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.