Anti-LIG4 / DNA Ligase 4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. LIG4 antibody detects DNA ligase 4, an essential enzyme that catalyzes the final ligation step in non-homologous end joining (NHEJ) DNA repair. The UniProt recommended name is DNA ligase 4 (LIG4). This enzyme joins DNA double-strand breaks generated during recombination, replication stress, or DNA damage, preserving genome stability.Functionally, LIG4 antibody identifies a 911-amino-acid ATP-dependent DNA ligase that seals nicks in double-stranded DNA by catalyzing phosphodiester bond formation. LIG4 operates in complex with XRCC4 and XLF/Cernunnos to align DNA ends and complete repair during NHEJ. It also plays a critical role in V(D)J recombination in lymphocytes, supporting immune diversity.The LIG4 gene is located on chromosome 13q33.3 and is ubiquitously expressed in proliferating tissues. Its expression is highest in immune cells, where DNA recombination occurs frequently. Proper function of LIG4 ensures efficient DNA repair and prevents chromosomal translocations and mutagenesis.Pathologically, mutations in LIG4 cause Ligase IV syndrome, characterized by microcephaly, developmental delay, immunodeficiency, and radiosensitivity. Deficiency in LIG4 impairs DNA double-strand break repair, leading to genomic instability and increased cancer susceptibility. Research using LIG4 antibody aids in studies of DNA repair, recombination, and genomic maintenance.LIG4 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect ligase enzymes in DNA damage response pathways. NSJ Bioreagents provides LIG4 antibody reagents optimized for DNA repair, oncology, and immunology research.Structurally, DNA ligase 4 contains an N-terminal DNA-binding domain, an adenylation domain that binds AMP, and a C-terminal XRCC4-interacting region necessary for NHEJ complex formation. This antibody enables detailed analysis of LIG4's enzymatic activity in genome integrity and repair pathways.