Anti-Interferon Regulatory Factor 6 (IRF6)

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:1:32,000, Immunohistochemistry: 2-3ug/ml in paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus, Western Blot: 0.2-0.6ug/ml, ~60kD band observed in Human Ovary lysates (calculated MW of 53.1kD according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55kD is observed. This band is not observed in the non-transfected HEK293, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.