Anti-Integrin, alpha5, beta3 (Vitronectin Receptor, CD51) (FITC)

In cell matrix adhesions, clustered integrins (each made up of a noncovalently linked alpha and beta subunit) bind to endothelium cell matrix (ECM) components via their globular head domains. They connect to the actin cytoskeleton via adaptor proteins that bind their short cytoplasmic tails. Cell matrix adhesions also act as signalling units by their capacity to organize the actin cytoskeleton and to accumulate various signalling intermediates. Integrin mediated adhesion regulates the actions of Rho-GTPases, which in turn affect cell-cell adhesion. The converse process can also take place: the activity of Rho-GTPases can be modulated by cadherins in adherens junctions. Besides mediating adhesion to ECM components, integrins participate in the assembly of an ECM. Alpha-V/beta-3 integrin is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Applications: Suitable for use in Flow Cytometry and Immunoassays.. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.