Anti-IKK alpha (Biotin) (Azide Free) (IkappaB Kinase alpha, IKKa, CHUK, Conserved Helix Loop Helix U

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1)(1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns (2,3). Applications: Suitable for use in Western Blot and immunoprepcipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1-2ug/ml. , Western Blot: 1ug/ml. Detects a band at ~85kD., Optimal dilution determined by the researcher. Positive Controls: Daudi, NIH 3T3, or Raw cells , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer., , Note: Applications are based on unconjugated antibody.