Anti-Histone H4S1Ph Monoclonal Antibody (RM194)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Phosphorylation of histone H4 at serine 1 (H4S1Ph) is increased during the S-phase and mitosis in HeLa cells, C. elegans, and Drosophila, as well as during spermatogenesis in mice. MG-63 cells expressing a serine-to-alanine substitution at Ser1 in histone H4, which abolishes its phosphorylation, have reduced markers of autophagy. Cayman's Histone H4S1Ph Monoclonal Antibody (RM194) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assays, and Western blot (WB) applications.Synonyms: H4pS1, H4pSer1, Histone H4 (Phospho-Ser1), Phospho-Histone H4 Serine 10, Phosphorylated Histone H4 Serine 1. Immunogen: Peptide corresponding to H4S1Ph. Clone Designation: RM194. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H4S1Ph, (+) H4S1Ph/R3Me1, (+) H4S1Ph/R3Me2, (-) Unmodified histone H4, (-) H2AS1Ph.