Anti-Histone H4R3Me1 Monoclonal Antibody (RM195)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Histone H4 can be monomethylated at arginine 3 (H4R3Me1) by protein arginine methyltransferase 1 (PRMT1), PRMT5, and PRMT6. Retinal levels of H4R3Me1 are reduced in a rat model of diabetes induced by streptozotocin (STZ, Cay-13104) compared with non-diabetic controls. Cayman's Histone H4R3Me1 Monoclonal Antibody (RM195) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Monomethylated Histone H4 Arginine 3. Immunogen: Peptide corresponding to H4R3Me1. Clone Designation: RM195. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H4R3Me1, (-) Unmodified H4R3, (-) H4R3Me2a, (-) H4R3Me2s, (-) H4R3Cit.