Anti-Histone H4K20Me3 Monoclonal Antibody (RM208)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Trimethylation of histone H4 at lysine 20 (H4K20Me3) is enriched at pericentric heterochromatin and is associated with gene silencing. In senescent cells, H4K20Me3 is enriched at senescence-associated heterochromatin foci (SAHF), and levels of H4K20Me3 are increased in senescent cells compared with proliferating cells. H4K20Me3 levels are elevated in tumor tissue, compared with adjacent non-tumor tissue, isolated from patients with esophageal squamous cell carcinoma, and elevated H4K20Me3 levels are associated with lymph node involvement and poor prognosis in these patients. Cayman's Histone H4K20Me3 Monoclonal Antibody (RM208) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.Synonyms: Trimethylated Histone H4 Lysine 20. Immunogen: Peptide corresponding to H4K20Me3. Clone Designation: RM208. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H4K20Me3, (+) H4K20Me2 (weakly), (-) Unmodified H4K20, (-) H4K20Me1, (-) H4K5Me1, (-) H4K12Me1.