Anti-GART / Phosphoribosylglycinamide formyltransferase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. GART antibody detects Phosphoribosylglycinamide formyltransferase, also known as trifunctional purine biosynthetic protein adenosine-3, encoded by the GART gene on chromosome 21q22.11. GART is a multifunctional enzyme of the de novo purine biosynthesis pathway, responsible for catalyzing three distinct steps in the conversion of phosphoribosylamine to inosine monophosphate (IMP). This trifunctional protein includes glycinamide ribonucleotide transformylase, aminoimidazole ribonucleotide synthase, and glycinamide ribonucleotide synthetase domains. By integrating multiple catalytic activities into a single polypeptide, GART coordinates metabolic flux through the purine biosynthetic pathway, ensuring efficient nucleotide production required for DNA, RNA, and energy metabolism.Functionally, GART is critical for rapidly dividing cells that require high nucleotide synthesis rates. The enzyme is localized in the cytoplasm and often organizes into dynamic purinosome complexes when purine demand is elevated. GART activity is tightly regulated, and its expression increases in proliferating cells, including embryonic tissues and tumors. Dysregulation of GART has been associated with cancer metabolism, where enhanced nucleotide biosynthesis supports uncontrolled proliferation. Additionally, its genomic location on chromosome 21 links GART to Down syndrome pathophysiology, since increased gene dosage contributes to altered purine metabolism in trisomy 21.Biochemically, GART catalyzes the formylation of glycinamide ribonucleotide using 10-formyltetrahydrofolate, forming formylglycinamide ribonucleotide, a crucial intermediate in IMP synthesis. Mutations or inhibition of GART disrupt purine biosynthesis, leading to impaired cell growth and potential embryonic lethality. Small molecule inhibitors of GART and other purinosome enzymes are being explored as antiproliferative agents in oncology.Experimentally, GART antibody is used to track enzyme expression in normal and cancerous tissues, evaluate purinosome assembly, and study folate-dependent metabolism. Applications include western blotting, immunofluorescence microscopy, and immunohistochemistry. In cancer research, GART antibody helps evaluate metabolic reprogramming, while in developmental biology it provides insights into nucleotide demand during embryogenesis. NSJ Bioreagents supplies GART antibody to support these diverse research fields, ensuring reliable detection of this multifunctional enzyme.