Anti-FTO / Fat mass and obesity associated protein, clone AEAA-6

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. FTO antibody targets Fat mass and obesity associated protein (FTO), a nuclear protein that functions as an RNA demethylase and plays an important role in post-transcriptional regulation of gene expression. FTO belongs to the AlkB family of dioxygenases and is primarily localized to the nucleus, where it associates with chromatin and RNA-processing machinery. Through its enzymatic activity, FTO removes methyl groups from specific RNA modifications, linking cellular metabolism to regulation of RNA stability, splicing, and translation. This positioning makes FTO a key molecular interface between nutrient status and gene expression control.Functionally, FTO is best known for its ability to demethylate N6-methyladenosine (m6A) and related RNA modifications, which are critical regulators of RNA fate. By modulating RNA methylation status, FTO influences transcript processing, turnover, and translational efficiency. These effects impact diverse cellular processes, including energy balance, cell growth, and differentiation. A FTO antibody supports studies focused on RNA epigenetics and the mechanisms by which RNA modifications regulate gene expression programs.FTO is broadly expressed across tissues, with notable expression in the brain, particularly in regions involved in energy homeostasis and feeding behavior. Its expression pattern reflects roles in both central nervous system regulation and peripheral metabolic tissues. Analysis of FTO localization and abundance provides insight into how RNA methylation dynamics respond to physiological cues such as nutrient availability and hormonal signaling. Because FTO activity is sensitive to cellular metabolic state, it is frequently examined in studies linking metabolism to gene regulatory mechanisms.From a biological and disease-relevance perspective, FTO has been extensively studied in the context of metabolic regulation and human disease. Genetic variation in the FTO locus has been strongly associated with obesity risk and body mass index in population studies, driving significant interest in its biological function. Beyond metabolism, FTO has also been implicated in cancer biology, neural development, and stress responses, where altered RNA methylation patterns can influence cell behavior. Investigating FTO expression and regulation contributes to understanding how RNA modification pathways participate in disease-associated phenotypes.At the molecular level, FTO is encoded by the FTO gene and produces a protein of approximately 58 kDa. The protein contains conserved catalytic motifs required for iron- and alpha-ketoglutarate-dependent demethylase activity. FTO function depends on proper nuclear localization and interaction with RNA substrates and regulatory cofactors. A FTO antibody supports research applications focused on RNA modification, metabolic signaling, and disease-related changes in gene regulation, with NSJ Bioreagents providing reagents intended for research use.