Anti-Estrogen Receptor alpha Rabbit Monoclonal Antibody (Clone RM292)

Estrogen receptor alpha (ERalpha) is a member of the nuclear receptor superfamily of transcription factors encoded by ESR1 in humans that has a key role in reproductive function and additional roles in cancer progression and inhibition. Alternative splicing of the ESR1 pre-mRNA produces one full-length 66 kDa isoform and two short-length 46 and 36 kDa isoforms, which contain truncated C-termini. ERalpha is comprised of an N-terminal domain that contains AF-1, which is critical for the transactivation function of ERalpha, a DNA-binding domain that recognizes estrogen response elements (EREs) on target genes, and a C-terminal ligand-binding domain (LBD) that contains the nuclear localization signal. ERalpha is widely expressed in numerous tissues, including breast, prostate, uterus, liver, and bone, and localizes to the cytoplasm in a complex with heat shock protein 90 (Hsp90). Upon estrogen stimulation, ERalpha dissociates from Hsp90, dimerizes, and translocates to the nucleus, where it binds EREs expressed by target genes encoding proteins that activate numerous signaling pathways, including ERK/MAPK, PI3K/Akt/mTOR, and NF-kappaB, and influence a variety of cellular processes, including reproductive function, bone homeostasis, and inflammation, as well as tumor progression and metastasis. ERalpha is overexpressed in more than 50% of patients with breast cancer, but is associated with improved prognosis, whereas loss of ERalpha is a feature of triple-negative breast cancer (TNBC) and is associated with resistance to hormonal therapy. Cayman's Estrogen Receptor alpha Rabbit Monoclonal Antibody (Clone RM292) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Immunogen: Peptide corresponding to a region near the N-terminus of human ERalpha. Clone Designation: RM292. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) ERalpha.