Anti-ERBB2 (Receptor Tyrosine-protein Kinase erbB-2, Proto-oncogene Neu, Proto-oncogene c-ErbB2, p18

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a 185kD type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain aa23-653 of 1256 shares 85% and 94% aa identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 110kD soluble fragment of ErbB2 from the cell surface. ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1, heuregulin 1) or NRG2. Applications: Suitable for use in Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells using 3T3-L-1 mouse embryonic fibroblast adipose-like cell line, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.