Anti-DNA Topoisomerase IIalpha (C-Term) Rabbit Monoclonal Antibody (RM394)

DNA topoisomerase IIalpha is a type II topoisomerase encoded by TOP2A. It is composed of an N-terminal ATPase domain, a central core domain containing a topoisomerase-primase (toprim) catalytic domain, winged helix domain, and a DNA-binding core, and a C-terminal domain subject to post-translational modifications. DNA topoisomerase IIalpha is expressed at the highest levels during the G2/M phase of the cell cycle. DNA topoisomerase IIalpha is involved in chromosome segregation and DNA replication, where it forms homodimers that induce DNA double-strand breaks in a DNA duplex, passes another DNA duplex through the cleavage, and then ligates the break. This process reduces DNA supercoiling and relieves torsional stress on the DNA. Due to the selective expression of DNA topoisomerase IIalpha during the proliferative phase of the cell cycle, it is a target for cancer therapeutics. Cayman's DNA Topoisomerase IIalpha (C-Term) Rabbit Monoclonal Antibody (RM394) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: DNA Topoisomerase 2alpha, TOP2A. Immunogen: Peptide from the C-terminal region of human DNA topoisomerase IIalpha. Clone Designation: RM394. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) DNA topoisomerase IIalpha.