Anti-CXCR3 (CD183), FITC conjugated, clone 2Ar1

CXCR3 is a cell surface chemokine receptor that belongs to the family of G protein linked, seven transmembrane domain containing receptors. With the cloning of this molecule, it was identified as the specific receptor for two CXC chemokines: gamma interferon induced protein and monokine induced by interferon gamma. The CXCR3 receptors appear to have affinity for CXC chemokines that do not contain the conserved ELR motif at their amino terminus and as a result IL-8 and platelet factor 4 (PF4) do not bind to this receptor. CXCR3 shares approximately 40% amino acid homology with the CXCR1 and CXCR2 receptors, and approximately 35% homology with the first five CCR receptors. Even though the two ligands for CXCR3 belong to the alpha chemokine family, IP-10 and MIG are the first chemokines to target lymphocytes. CXCR3 molecules were also found by mRNA expression on activated T cells, B cells and NK cells, but not on monocytes, resting T cells or neutrophils. Of clinical interest is the demonstration of CXCR3 expression on cells from B cell chronic lymphocytic leukemias and splenic marginal zone lymphomas. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul/1x10e5 cells to detect human CXCR3., Optimal dilutions to be determined by the researcher. Hybridoma: Balb/c mouse inoculated with hCXCR3 transfected NS0 mouse myeloma cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.