Anti-Csk (CSK, c-src tyrosine kinase)

Alternate Name: CSK, c-src tyrosine kinase, Domain Information: Eukaryotic protein kinase domain, SH3 domain, Serine/Threonine protein kinases, catalytic domain, Src homology 2 domains, Src homology 3 domains, Src homology domain 2, Tyrosine kinase, catalytic domain , Gene Ontology Terms: protein C-terminus binding activity, protein tyrosine kinase activity, protein amino acid phosphorylation, regulation of cell cycle, cytoplasm, Application(s): Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 50kD band observed in Jurkat lysate (predicted MW of 50.7kD according to NP_004374.1). Recommended for use at 0.5-2ug/ml. Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. , Immunohistochemistry: In paraffin embedded Human Tonsil, strong staining of germinal centre cells and less intense labelling in the interfollicular area. Recommended concentration 0.1-0.3ug/ml, , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C7945-05D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.