Anti-Cortactin (EMS1, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 sr

Alternate Name: EMS1, ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate), Cttn, CORTACTIN, oncogene EMS1, Domain Information: Repeat in HS1/Cortactin, Src homology 3 domains , Gene Ontology Terms: soluble fraction, peripheral plasma membrane protein, cytoskeleton, Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested. Western blot: Approx 75-80kD band seen in A431 cell lysate. Recommended for use at 0.5-2ug/ml. Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C7901-83B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.