Anti-CD51 (Integrin alphaV, Vitronectin alpha Subunit Receptor, VNR), clone 2Q895

CD51 represents the 150kD integrin alphaV chain that associates with integrin beta3 (CD61) to form the CD51/CD61 vitronectin receptor on endothelial cells, certain activated leukocytes, NK cells, macrophages, neutrophils and platelets. CD51 is the most promiscuous integrin a subunit, it can form heterodimers with the beta1 (CD29), beta3 (CD61), beta5, beta6 and beta8 subunits in various tissues. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. It also mediates leukocyte-endothelial cell adhesion via interaction with CD31.1-4, Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Immunohistochemistry :Frozen sections, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol , aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,