Anti-CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc), clone 3H1512

CD49f is expressed by platelets, weakly by monocytes and by a subset of lymphocytes. CD49f is also widely expressed on epithelial tissues. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10-1:50. Use 10ul of suggested dilution to label 10e6 cells in 100ul. , Immunohistochemistry (frozen): 1:50-1:100, Optimal working dilutions to be determined by researcher. Recommended Positive Control Tissue (IHC): Tonsil, Recommended Negative Controls: Mouse IgG1 Negative Control, Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG:HRP, F(ab')2 rabbit anti mouse IgG:RPE conjugate, F(ab')2 rabbit anti-mouse IgG:FITC, Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.