Anti-CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc)

Human CD49f molecules are integrin alpha 6 chains that are expressed as heterodimers with CD29 (beta1integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml, Immunohistochemistry: frozen, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5 x 10e5 cultured Raji human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2405-04N at 10ug/ml., 2. Cells were washed twice and incubated with I1904-06F., 3. Cells were washed three times, fixed and analyzed by FACS. , 4. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a mouse IgG1 negative control (I1904-78) a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.