Anti-CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc), clone 2B2.33

CD49f is the 150kD integrin alpha6 chain which associates noncovalently with the integrin beta1 subunit (CD29) and with the integrin beta4 (CD104) chain to form, respectively, the very late antigen-6 (VLA-6) and alpha6beta4 integrin heterodimers. VLA-6 is expressed on thymocytes, T lymphocytes, monocytes and platelets. CD49f/CD29 expression is upregulated on activated T lymphocytes and memory T cells. VLA-6 serves as the laminin receptor on platelets, monocytes and T cells. VLA-6-mediated binding to laminin provides a costimulatory signal to T cells for activation and proliferation. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: < 1ug/10e6 cells, Immunohistochemistry: frozen sections, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.