Anti-CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha

CD49d (VLA-4) inhibits adhesion of rat lymphocytes to cytokine-activated endothelial cells. It blocks binding of rat lymphocytes to purified VCAM-1 and to the CS-1 peptide of fibronectin. VLA-4 is a leukocyte integrin which plays an important role in lymphocyte adhesion and activation. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:40, 10ul labels 10e6 cells in 100ul , Immunohistochemistry (Frozen): 1:50-1:100. Overnight incubation with C2404-46G2 and acetone fixation for frozen sections is recommended. Not suitable for use with paraffin sections., Optimal dilutions to be determined by the researcher. Hybridoma: SP2.0-Ag14 myeloma cells with spleen cells from Balb/c mice, Positive Control for Immunohistochemistry: Human tonsil, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.