Anti-CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL)

Human CD11a (alpha L integrin) complexes with CD18 (beta2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1, 2, and 3, also designated CD54, CD102, and CD50 respectively. LFA-1 is expressed on lymphocytes, monocytes and neutrophils and plays a role in cell-cell adhesion. Applications: Suitable for use in Flow Cytometry, Western Blot and Antibody Blocking. Other applications not tested. Recommended Dilution: Antibody blocking: 5-10ug/ml blocks binding of ICAM-1 and ICAM-3 to LFA-1., Flow cytometry: 5ug/ml, Optimal dilutions to be determined by the researcher. Flow cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2262-02C at 5ug/ml. , 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC. , 3. Cells were then washed three times, fixed and analyzed by FACS.   , 4. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,