Anti-CAR (Coxsackie Adenovirus Receptor), clone 2T6

The coxsackie virus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation. Applications: Suitable for Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500 detects CAR on 10ug of mouse small intestine lysates. Mouse small intestine lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with C1079. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system., Immunocytochemistry: 1-2ug/ml shows positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X., Optimal dilutions to be determined by the researcher. Positive Control: Lung tissue, Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.